A multi-center study on the percentage of relative abundance, of Klebsiella pneumoniae and Klebsiella oxytoca ,socio-demographic profile and clinical parameters of oral squamous carcinoma tissues of a group of Sri Lankan male patients

Background: Emergence of carbapenemase producing Klebsiella pneumoniae and asymptomatic oral colonization within human populations has become a public health threat Enrichment of oral Klebsiella species in neoplastic remain unclear. Aim: To find out the percentage of relative abundance, of Klebsiella pneumoniae and Klebsiella oxytoca,socio-demographic profile and clinical parameters of oral squamous carcinoma tissues of a group of Sri Lankan male patients in multi-centers. Method: A group of male patients comprising 25 OSCC cases visiting 9 OMF units in 6 Provinces in Sri Lanka represented the vast majority of OSCC patients.  DNA extracted from fresh biopsies was sequenced for the V1 to V3 region with Illumina’s 2 × 300–bp chemistry. High-quality nonchimeric merged reads were classified to the species level with a prioritized BLASTN-based algorithm and identified upto species level.  Downstream compositional analysis was performed with QIIME (Quantitative Insights into Microbial Ecology)  Results: K. pneumoniae was presented an average relative abundance of 1.45%, significantly higher than K. oxytoca, which has a relative abundance of 0.03%. Conclusion: K. pneumoniae over growth was seen compared to K. oxytoca in OSCC cases. This possess an emerging threat of transmitting this pathogen in the community and heath care associated environments as the oral and nasal cavities act as ideal niche for nourishment and flourishment of this opportunistic pathogen, notorious for nosocomial infections and multi drug resistance.