Pnrc2 promotes rapid mRNA decay and coordinately supports early development with P-body factors Ddx6 and Ddx61

Somitogenesis, the sequential segmentation of vertebrate embryonic mesoderm, is controlled by the segmentation clock, a molecular oscillator that controls periodic gene expression in unsegmented mesoderm and is regulated by a negative feedback loop driven by Hes/Her transcriptional repressors. In zebrafish, Pnrc2 is required for decay ofher1transcript and additional oscillatory gene transcripts. Despite accumulation of numerous mRNAs including those encoding key developmental regulators, overt embryonic phenotypes are absent inpnrc2mutants. Our previous work suggested that accumulated mRNAs are not translated inpnrc2mutants, though the underlying mechanism(s) was unknown. We show here that many overexpressed transcripts inpnrc2mutants have shortened poly(A) tails and are disengaged from ribosomes, and that deadenylation inhibition leads to somite defects inpnrc2mutants. In contrast, transcripts encoding the P-body protein, Ddx61, are both overexpressed and engaged with ribosomes, leading to an increase in Ddx61 protein. Co-depletion of Ddx61 and its ohnolog Ddx6 enhancesher1accumulation and later leads to strong morphological defects inpnrc2mutants. Together, our results show that multiple post-transcriptional mechanisms ensure proper translation when mRNA decay is inhibited.