PYEAST – Python Enabled Automated Strain Transformation

Saccharomyces cerevisiaeis a widely used biotechnological workhorse in both academic and industrial settings. One reason for its continued popularity is the extensive legacy of genetic tools, developed over its long history of use, that enable precise manipulation of theS. cerevisiaegenome. These tools have enabled extensive genetic characterisation and dramatic re-programming efforts for applications ranging from fundamental research to industrial chemical production. Here we present a digital toolkit called PYEAST (<underline>Py</underline>thon<underline>E</underline>nabled<underline>A</underline>utomated<underline>S</underline>train<underline>T</underline>ransformation) that encodes some of the most widely used methods for working withS. cerevisiaeand modernizes them to leverage advances in DNA synthesis.