Targeted sex ratio distortion in the mouse
Abstract
In animal breeding, female or male progeny is preferred depending on the use of the animals. Especially in livestock, offspring with undesired sex is of low economic value and often culled. Here we achieve targeted sex ratio distortion in the mouse, leveraging components of a selfish supergene, thet-haplotype. Utilizing its central element, thet-responder, we generate a highly “selfless” genetic element disabling transgenic sperm. When integrated on the X or Y chromosome it results in a high prevalence of non-transgenic offspring of the desired sex.
Our strategy overcomes key limitations of existing strategies, including compromised animal health, reduced fertility, and fully transgenic breeding stocks, which might foster acceptance of its application to livestock. The efficacy of our approach marks a groundbreaking advancement in animal breeding.
Graphical abstract
<fig id="ufig1" position="float" fig-type="figure" orientation="portrait"><graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="660540v1_ufig1" position="float" orientation="portrait"/></fig>Transgene integration on X or Y chromosome affects the motility of transgenic sperm resulting in a strong sex-bias towards non-transgenic/wildtype offspring. Application to livestock can reduce unwanted progeny and transform farm animal breeding.
Related articles
Related articles are currently not available for this article.