Effects of Lactobacillus plantarum P10-R10, Lactobacillus salivarius P11-R11 and Lactobacillus reuteri DSM17938 on intestinal microbiota and plasma metabolism in mice with alcohol-related liver injury

Background Previous research has identified alterations in the Lactobacillus genus in alcohol-related liver disease, observed in both clinical and murine experiments. Different species of Lactobacilli exhibit distinct functions across various disease states. Understanding how different Lactobacillus species influence alcohol-related liver disease could lead to targeted therapeutic interventions that harness beneficial bacteria or mitigate harmful effects. Additionally, exploring these relationships may provide insights into personalized approaches for managing liver health in individuals with varying microbial compositions. Method An alcohol-related liver injury mice model was established based on Lieber-DeCarli model and Gao-Binge model. Three species of lactobacillus were administered for intervention, and control and model groups were set up. Samples were collected after 28 days, and the effects on liver function, intestinal microbiota and metabolites were evaluated by detecting indicators. Results Lactobacillus plantarum P10-R10 could ameliorate alcohol-induced liver injury. After the intervention of Lactobacillus plantarum P10-R10, plasma AST decreased in mice, liver oil red indicated a decrease in the degree of steatosis, and TG decreased in liver tissue. Relative abundance of beneficial bacteria such as Lactiplantibacillus, Faecalibacterium and Bifidobacterium increased while the relative abundance of harmful bacteria such as Helicobacter and Klebsiella decreased. L-carnitine and theophylline increased in plasma and were positively correlated with elevated genus. Functional implications of Lactobacillus salivaria P11-R11 and Lactobacillus reuteri DSM17938 merit further exploration. Conclusions The role of different species of Lactobacillus in ALD is different, and Lactobacillus plantarum ameliorate liver injury in mice with ALD.