New γ-pyrone derivative from Helichrysum sanguineum (L.) and its Anti-Helicobacter pylori activity

Helichrysum sanguineum is a medicinal plant traditionally used for treatment of several diseases. Phytochemical investigation of its aerial parts led to isolation of four compounds, including one new γ -pyrone derivative named Kadipyrone (1) and identified as 2-(4-hydroxy-6-methylheptan-2-yl)-3-methyl-4H-pyran-4-one. Three known compounds were also isolated including luteolin (2), quercetin (3) and para hydroxy benzoic acid (4). Structures of these compounds were elucidated through extensive spectroscopic methods, including ESI-MS, HR-ESI-(qTOF)-MS, ¹H NMR,¹³C-NMR, APT-NMR experiments and two dimensional NMR techniques (HSQC and HMBC).Further, different fractions obtained from Helichrysum sanguineum and compound 1 were evaluated for their anti-bacterial activity against Helicobacter pylori through diffusion assay. The results indicated that compound 1 has a promising activity toward H. pylori with a minimum inhibitory concentration (MIC) of 32 µg/ml, suggesting the potential role of compound 1 in the treatment of H. pylori infection.